Project 03: The role of L-selectin in sterile injury
PI London: A. Ivetic; PI Göttingen: D.M. Katschinski; PhD student: S.J. Hart - finished PhD
Neutrophils are the first leukocyte subset to accumulate within the myocardial parenchyma post-MI, with cell numbers peaking at 24 – 72 hours following the onset of ischaemia. On activation, neutrophils express a dominantly pro-inflammatory phenotype, releasing reactive oxygen species; secreting proteolytic and matrix-degrading enzymes; and engaging in phagocytosis and extracellular trap formation (NETosis). Neutrophils are known to drive MI pathogenesis, for example by promoting infarct expansion and cardiac rupture.
The PhD student aims to identify the molecular mechanisms which underpin neutrophil activation in the infarcted heart, and highlight novel points for future therapeutic intervention.
Research Methods:
- In vivo surgery
- Echocardiography
- Tissue Harvesting
- Immunohistochemistry
- FACS and Flow Cytometry
- Next-Generation Imaging
Speaker British Heart Foundation Centre of Research Excellence
Research interests: Mechanisms of inflammation
Speaker IRTG 1816
PhD student IRTG 1816, KCL
Current Position: Trainee Patent Attorney, Mewburn Ellis LLP, London, UK